Traditional Medicine and Ethnomedicine World Conference 2026

Abstract

Plasmid mediated antimicrobial resistance in Salmonella spp. is a growing global concern. Plant-derived agents have been proposed as alternative strategies for plasmid curing. This study comparatively assessed the plasmid curing efficacy of Curcuma longa and Guiera senegalensis extracts. Forty Salmonella clinical isolates were obtained from General Hospital Azare, Bauchi State, Northern Nigeria and confirmed phynotypically using API 20E kits and Genotypically using PCR amplification. Antimicrobial susceptibility testing was conducted using the Kirby Bauer disc diffusion method. Multidrug resistance isolates were subjected to  plasmid profiling via agarose gel electrophoresis. All isolates encoding plasmid were treated with different  concentrations of  the plant extracts The plasmid curing potentials of the extract were ascertained by exposing the resistance isolates to different concentrations (1 to 8 percent) of the extracts, and then followed by susceptibility test after curing. Plasmid loss and changes in resistance profiles were evaluated  after post treatment using Gel electrophoresis. Statistical analysis was performed using one way ANOVA at (p lesser than 0.05). Among the isolates, 28 (70 percent) were Multidrug resistance, and 24 (85.7percent) carried plasmids. Curcuma longa treatment reversed the susceptability to 21 (75percent) of isolates, while Guiera senegalensis reversed 58.3percent. Both extracts significantly reduced antibiotic resistance, with Curcuma longa showing superior activity at (p lesser 0.05).

Keywords Antimicrobial susceptibility profile, Multidrug resistance, Plasmid curing, plasmid mediated resistanc e and polymerase chain reaction.